In December 2023, Warner Bros. revealed the official cast list for "Suicide Squad ISEKAI," and it's a who's-who of voice acting talent: Anna Nagase, Yuichiro Umehara, Reigo Yamaguchi, Takehito Koyasu, Jun Fukuyama, and Subaru Kimura are starring as the titular team.

Despite landing what is arguably the lead role in Harley Quinn, Anna Nagase is the least-experienced member of the main voice cast. She's best known for voicing Ushio Kofune in the highly-rated time travel anime series "Summer Time Rendering," though she has also worked on shows such as "Jujutsu Kaisen" and "Cyberpunk: Edgerunners." The Joker is far from the first intimidating character that Yuichiro Umehara has voiced: He plays the eponymous character in the gory series "Goblin Slayer," which returned for a second season in 2023. He also recently played Jugram Haschwalth in "Bleach: Thousand-Year Blood War."

Reigo Yamaguchi, who is playing Deadshot, has plied his trade on the likes of "Food Wars: Shokugeki no Soma," "One Punch Man," and "Dr. Stone," while Takehito Koyasu, who is playing Peacemaker, is known to fans as the voice of Dio in "JoJo's Bizarre Adventure" and Zeke in "Attack on Titan." Jun Fukuyama (who plays a very suave-looking Clayface) is famous for the roles of Lelouch Lamperouge in "Code Geass: Lelouch of the Rebellion" and Koro-sensei in "Assassination Classroom," and Subaru Kimura (who features as a denim dungaree-wearing King Shark) recently voiced Sakuragi Hanamichi in the hit anime movie "The First Slam Dunk."